Premium
Both osteopontin‐c and osteopontin‐b splicing isoforms exert pro‐tumorigenic roles in prostate cancer cells
Author(s) -
Tilli Tatiana M.,
Mello Kivvi D.,
Ferreira Luciana B.,
Matos Aline R.,
Accioly Maria Thereza S.,
Faria Paulo A.S.,
Bellahcène Akeila,
Castronovo Vincent,
Gimba Etel R.
Publication year - 2012
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.22523
Subject(s) - osteopontin , prostate cancer , cancer research , tumor progression , biology , alternative splicing , cell growth , rna splicing , pi3k/akt/mtor pathway , gene isoform , cancer , signal transduction , microbiology and biotechnology , immunology , gene , genetics , rna
BACKGROUND Alternative splicing of the osteopontin ( opn , spp 1) gene generates three protein splicing isoforms (OPN‐SI), designated as OPNa, OPNb, and OPNc, which have demonstrated specific roles in different tumor models. This work aims to investigate the roles of each OPN‐SI in prostate cancer (PCa) progression by using in vivo and in vitro functional assays. METHODS The expression levels of OPN‐SI in prostate cell lines were analyzed by qRT‐PCR. PC‐3 was stably transfected with expression vectors containing OPNa, OPNb, and OPNc, as well as empty vector controls. PC‐3 cells overexpressing each construct were analyzed for in vivo tumor growth and in relation to different aspects mimicking tumor progression, such as cell proliferation, migration, invasion, and soft agar colony formation. RESULTS OPN‐SI are overexpressed in PCa as compared to non‐tumoral prostate cell lines. OPNc and OPNb overexpressing cells significantly activated enhanced xenograft tumor growth and PC‐3 proliferation, migration, invasion, and soft agar colony formation, as well as the expression of MMP‐2, MMP‐9, and VEGF. These isoforms also support sustained proliferative survival. We found that both OPNc and OPNb pro‐tumorigenic roles are mainly mediated through PI3K signaling. Inhibition of this pathway by using LY294002 specifically inhibited tumor progression features evoked by OPNc and OPNb overexpression. CONCLUSIONS Our data provide evidence that both OPNc and OPNb splicing isoforms promote distinct aspects of PCa progression by inducing PI3K signaling. These data give support to strategies aiming to downregulate OPNc and OPNb expression as an approach to inhibit PCa progression. Prostate 72:1688–1699, 2012. © 2012 Wiley Periodicals, Inc.