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RECK overexpression decreases invasive potential in prostate cancer cells
Author(s) -
Rabien Anja,
Ergün Bettina,
Erbersdobler Andreas,
Jung Klaus,
Stephan Carsten
Publication year - 2011
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.21498
Subject(s) - lncap , prostate cancer , matrix metalloproteinase , metastasis , zymography , cancer research , angiogenesis , prostatectomy , prostate , blot , cancer , cell growth , medicine , pathology , biology , gene , biochemistry , genetics
Abstract BACKGROUND RECK is a tumor suppressor which inhibits metastasis and angiogenesis. Based on RECK expression in prostate cancer tissue and cell lines, our aim was to investigate functional relevance of RECK for prostate carcinoma. METHODS RECK protein levels were determined by Western blotting in the human prostate cell lines BPH‐1, DU‐145, LNCaP, PC‐3, and in tissue of 12 normal/tumor matches of patients after radical prostatectomy. Functional characteristics of DU‐145 cells with stable RECK overexpression included proliferation, invasion, regulation of matrix metalloproteinases MMP‐2, MMP‐9, and MMP‐14 measured by zymography (MMP‐2 and ‐9) or commercially available assays. RESULTS RECK was expressed in cell lines and tissue with a significant decrease in malignant tissue ( P = 0.002). RECK overexpression caused an up to 80% decrease in invasion for DU‐145 cells ( P < 0.001) and a decrease of pro‐MMP‐9 (42%) and of pro‐/active MMP‐14 (up to 53% of control). Proliferation was not affected by RECK overexpression. CONCLUSIONS The considerable anti‐invasive potential of RECK points to new therapeutic possibilities for prostate cancer. Prostate 72:948–954, 2012. © 2011 Wiley Periodicals, Inc.