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Elevated expression of cancer‐associated proliferating cell nuclear antigen in high‐grade prostatic intraepithelial neoplasia and prostate cancer
Author(s) -
Wang Xiaoyan,
Hickey Robert J.,
Malkas Linda H.,
Koch Michael O.,
Li Lang,
Zhang Shaobo,
Sandusky George E.,
Grig David J.,
Eble John N.,
Cheng Liang
Publication year - 2010
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.21291
Subject(s) - intraepithelial neoplasia , prostate cancer , prostate , proliferating cell nuclear antigen , adenocarcinoma , medicine , prostatectomy , pathology , cancer , high grade prostatic intraepithelial neoplasia , antigen , antibody , prostate specific antigen , pca3 , immunohistochemistry , immunology
BACKGROUND Proliferating cell nuclear antigen (PCNA) plays an important role in DNA replication and repair. The expression and potential utility of this marker in prostatic neoplasia is uncertain. With the development of this new caPCNA selective antibody, we explored the potential utility of this marker in prostate cancer. METHODS Using a traditional primary Fab2' rabbit anti‐caPCNA antibody‐HRP conjugated secondary anti‐Fab2' antibody format, the expression of the caPCNA was analyzed in prostate tissue from 89 radical prostatectomy specimens. The caPCNA expression was correlated with clinicopathologic characteristics. RESULTS The fraction of cells staining positively with caPCNA antibody in prostatic adenocarcinoma (mean, 23%) was significantly higher than that in benign prostatic epithelium (mean, 2%; P < 0.001) or high‐grade prostatic intraepithelial neoplasia (PIN) (mean, 6%; P < 0.05). Moreover, the intensity of caPCNA expression in prostatic adenocarcinoma (mean, 2.9) was significantly higher than that in benign prostatic tissue (mean, 0.7; P < 0.001) or high‐grade PIN (mean, 2.0; P < 0.001). Benign prostatic epithelium showed only minimal or negative reactivity. There was significant correlation between the percentage of caPCNA expression and primary Gleason grade ( P = 0.01), and with Gleason score ( P = 0.02). Adenocarcinomas with positive vascular invasion had a significantly higher percentage of cells staining with caPCNA antibody ( P < 0.0001) and a higher intensity of caPCNA expression ( P = 0.04). CONCLUSIONS Our data indicate that increased expression of the cancer‐associated isoform of PCNA is common in prostatic adenocarcinoma and its precursor and may be a useful biomarker. Prostate 71:748–754, 2011. © 2010 Wiley‐Liss, Inc.