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Phorbol ester phorbol‐12‐myristate‐13‐acetate induces epithelial to mesenchymal transition in human prostate cancer ARCaP E cells
Author(s) -
He Hui,
Davidson Alec J.,
Wu Daqing,
Marshall Fray F.,
Chung Leland W.K.,
Zhau Haiyen E.,
He Dalin,
Wang Ruoxiang
Publication year - 2010
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.21146
Subject(s) - protein kinase c , vimentin , epithelial–mesenchymal transition , stromal cell , phorbol , cancer research , biology , cancer cell , signal transduction , chemistry , microbiology and biotechnology , medicine , cancer , immunology , transition (genetics) , biochemistry , gene , immunohistochemistry
BACKGROUND We have reported that human prostate cancer ARCaP E cells undertake epithelial to mesenchymal transition (EMT) when stimulated by certain soluble factors, and that EMT is regulated by surface receptor‐elicited signaling pathways through protein phosphorylation. It is known that phorbol ester phorbol‐12‐myristate‐13‐acetate (PMA), a potent antagonist to both conventional and novel protein kinase C (PKC) isoenzymes, induces cancer cell scattering. METHODS To assess the effect of PMA on EMT, ARCaP E cells were treated with PMA and were assayed for EMT‐related morphologic and behavioral changes. Specific inhibitors were used to investigate the PMA‐induced EMT. RESULTS PMA at 100 nM induced EMT in a time‐dependent manner, resulting in a complete change from epithelial to mesenchymal stromal morphology. Concurrently, PMA inhibited expression of epithelial marker E‐cadherin and increased the level of stromal marker protein vimentin, while the treated cells showed increased migratory and invasive capacities. Using specific inhibitors, we confirmed that the effect of PMA was mediated by PKC, while isoenzymes of the novel PKC subfamily were implicated as the main mediator. Finally, we determined that the EMT was dependent on newly synthesized proteins, because inhibitors for gene transcription and protein translation could both inhibit the initiation of EMT. CONCLUSIONS Although PMA is well known for its effects on cell migration and tumor formation, this work is the first to define PMA as an EMT inducer in prostate cancer cells. Further investigation in this experimental model may reveal important regulatory mechanisms and additional molecular changes underlying EMT. Prostate 70: 1119–1126, 2010. © 2010 Wiley‐Liss, Inc.