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Effect of intermittent fasting with or without caloric restriction on prostate cancer growth and survival in SCID mice
Author(s) -
Buschemeyer W. Cooper,
Klink Joseph C.,
Mavropoulos John C.,
Poulton Susan H.,
DemarkWahnefried Wendy,
Hursting Stephen D.,
Cohen Pinchas,
Hwang David,
Johnson Tracy L.,
Freedland Stephen J.
Publication year - 2010
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.21136
Subject(s) - medicine , prostate cancer , endocrinology , prostate , intermittent fasting , cancer , caloric theory , caloric intake , body weight
Caloric restriction (CR) delays cancer growth in animals, though translation to humans is difficult. We hypothesized intermittent fasting (i.e., intermittent extreme CR), may be better tolerated and prolong survival of prostate cancer (CaP) bearing mice. METHODS We conducted a pilot study by injecting 105 male individually‐housed SCID mice with LAPC‐4 cells. When tumors reached 200 mm 3 , 15 mice/group were randomized to one of seven diets and sacrificed when tumors reached 1,500 mm 3 : Group 1: ad libitum 7 days/week; Group 2: fasted 1 day/week and ad libitum 6 days/week; Group 3: fasted 1 day/week and fed 6 days/week via paired feeding to maintain isocaloric conditions to Group 1; Group 4: 14% CR 7 days/week; Group 5: fasted 2 days/week and ad libitum 5 days/week; Group 6: fasted 2 day/week and fed 5 days/week via paired feeding to maintain isocaloric conditions to Group 1; Group 7: 28% CR 7 days/week. Sera from mice at sacrifice were analyzed for IGF‐axis hormones. RESULTS There were no significant differences in survival among any groups. However, relative to Group 1, there were non‐significant trends for improved survival for Groups 3 (HR 0.65, P = 0.26), 5 (0.60, P = 0.18), 6 (HR 0.59, P = 0.16), and 7 ( P = 0.59, P = 0.17). Relative to Group 1, body weights and IGF‐1 levels were significantly lower in Groups 6 and 7. CONCLUSIONS This exploratory study found non‐significant trends toward improved survival with some intermittent fasting regimens, in the absence of weight loss. Larger appropriately powered studies to detect modest, but clinically important differences are necessary to confirm these findings. Prostate 70: 1037–1043, 2010. © 2010 Wiley‐Liss, Inc.