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A novel, spontaneously immortalized, human prostate cancer cell line, Bob, offers a unique model for pre‐clinical prostate cancer studies
Author(s) -
Attard Gerhardt,
Rizzo Sian,
Ledaki Ioanna,
Clark Jeremy,
Reid Alison H.M.,
Thompson Alan,
Khoo Vincent,
de Bono Johann S.,
Cooper Colin S.,
Hudson David L.
Publication year - 2009
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20997
Subject(s) - prostate cancer , prostate , cancer , medicine , cancer cell lines , immortalised cell line , cell culture , cancer research , oncology , pathology , cancer cell , biology , genetics
New in vitro models of castration‐resistant prostate cancer (CRPC) are urgently required. METHODS Trans‐rectal needle biopsies (TRBP) of the prostate were performed for research purposes on progressing CRPC patients who had not received prior treatment to the prostate. Biopsies were immediately digested with collagenase and plated onto collagen‐coated flasks with a feeder layer of 3T6 cells and cultured in cytokine‐supplemented keratinocyte serum‐free medium. RESULTS Biopsies from 25 patients were collected and one of these, following an initial period of crisis, spontaneously immortalized. A series of cell lines called Bob were then established from a clone that survived CD133‐selection followed by 4 weeks under adhesion‐independent conditions in methylcellulose. Gains and losses previously described in clinical prostate tumors, most notably loss of 8(p) and gain of 8(q), were identified on comparative genomic hybridization and long‐term growth in culture, survival in methylcellulose and invasion through matrigel confirmed the malignant phenotype of Bob. Furthermore, Bob expressed high levels of p53 and markers of early differentiation, including K8, prostatic acid phosphatase and prostate stem cell antigen. There was, however, no in vivo growth and ERG and ETV1 were not rearranged. Growth in serum permitted some differentiation. CONCLUSION This is the first spontaneously immortalized prostate cancer cell line to be established from a TRBP of a patient with CRPC. Bob is a novel pre‐clinical model for functional studies in CRPC and especially for studying the CRPC “basal” phenotype. Prostate 69: 1507–1520, 2009. © 2009 Wiley‐Liss, Inc.