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Botulinum Toxin Type A Inhibits the Growth of LNCaP Human Prostate Cancer Cells In Vitro and In Vivo
Author(s) -
Karsenty Gilles,
Rocha Joice,
Chevalier Simone,
Scarlata Eleonora,
Andrieu Claudia,
Zouanat Fatima Z.,
Rocchi Palma,
Giusiano Sophie,
Elzayat Ehab A.,
Corcos Jacques
Publication year - 2009
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20958
Subject(s) - lncap , prostate cancer , in vivo , prostate , apoptosis , medicine , cell growth , cancer research , growth inhibition , endocrinology , cancer , pathology , biology , biochemistry , genetics , microbiology and biotechnology
AND OBJECTIVE Botulinum toxin type A (BTA) intraprostatic injection induces an improvement of urinary symptoms related to benign prostatic hypertrophy (BPH). Infra‐clinical prostate cancer (PCa) foci and pre‐neoplasic lesions occur concomitantly with BPH in a significant number of patients. The objective of this study was to address whether BTA influences the growth of prostate tumors. METHODS Proliferation of PC‐3 and LNCaP cell lines exposed or not to BTA (Botox) was assessed and compared. Presence of synaptic vesicle 2 (SV2) protein, the membrane receptor of BTA, was studied in both cell lines. After nude mice bearing LNCaP xenografts received intra‐tumoral BTA or saline injection, tumor volume, serum PSA, histopathology and detection of apoptosis were comparatively assessed. RESULTS BTA significantly reduced LNCaP cell proliferation and increased apoptosis in a dose‐dependent manner but did not affect PC‐3. The SV2 receptor was present in both cell lines at a ratio of 4:1 (LNCaP/PC‐3). One unit of BTA resulted in a significantly lower growth rate and slower PSA progression over 28 days compare to controls. The tumors were morphologically similar. There were significantly more apoptotic cells compared to controls. CONCLUSION BTA inhibits the growth of LNCaP human PCa cells in vitro and in vivo. These findings indicate that intra‐prostatic BTA injections to treat BPH are unlikely to promote the growth of co‐existing infra‐clinical PCa foci in men. A potential inhibitory effect of BTA on the growth of human PCa should be further studied. Prostate 69:1143–1150, 2009. © 2009 Wiley‐Liss, Inc.