Premium
Molecular analysis of multifocal prostate cancer by comparative genomic hybridization
Author(s) -
Kobayashi Masayuki,
Ishida Haruna,
Shindo Takayuki,
Niwa Shinichiro,
Kino Mika,
Kawamura Koji,
Kamiya Naoto,
Imamoto Takashi,
Suzuki Hiroyoshi,
Hirokawa Yoshifumi,
Shiraishi Taizo,
Tanizawa Tohru,
Nakatani Yukio,
Ichikawa Tomohiko
Publication year - 2008
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20832
Subject(s) - prostate cancer , prostate , prostatectomy , comparative genomic hybridization , microdissection , pathology , cancer , laser capture microdissection , medicine , biology , cancer research , gene , genetics , chromosome , gene expression
BACKGROUND Prostate cancer is often multifocal and shows histological heterogeneity among different tumor foci within the same prostate. We analyzed the origin and molecular basis of multifocal prostate cancer and genomic alterations associated with tumor progression. METHODS We examined 45 multifocal prostate cancer foci from 22 radical prostatectomy specimens by comparative genomic hybridization (CGH). Laser capture microdissection (LCM) was used to gather cancer cells from frozen prostate specimens. RESULTS Frequent chromosomal alternations were losses of 2q21–24 (22.2%), 6q14‐22 (60.0%), 8p12–22 (35.6%), 13q14–31 (44.4%) and 16q13–24 (24.4%) and gains of 8q21.3–24.3 (37.8%) and 7q21–33 (20.0%). Frequency of losses of 8p12–22 and 16q13–24 and gains of 8q21.3–24.3 were significantly higher in tumors with high Gleason score (GS) than in those with low GS ( P < 0.01, P < 0.05, and P < 0.01, respectively). Tumors with losses of 8p12–22 or 13q14–31 displayed larger volume than those without such losses ( P < 0.05 and P < 0.01, respectively). In comparison between different tumor foci within the same prostate, chromosomal alterations did not show completely the same pattern between any tumor foci, except for one case in which two of the three foci displayed no chromosomal abnormalities. More malignant tumors (high GS or extracapsular invasion) displayed significantly higher frequency of losses of 8p12–22 ( P < 0.05). CONCLUSIONS These results suggest that tumor foci within the same prostate represent independent tumors with differing clonal origin and that loss of 8p12–22 represents an important determinant of prostate cancer progression. Prostate © 2008 Wiley‐Liss, Inc.