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A methyl‐deficient diet modifies histone methylation and alters Igf2 and H19 repression in the prostate
Author(s) -
Dobosy Joseph R.,
Fu Vivian X.,
Desotelle Joshua A.,
Srinivasan Rajini,
Kenowski Michelle L.,
Almassi Nima,
Weindruch Richard,
Svaren John,
Jarrard David F.
Publication year - 2008
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20782
Subject(s) - epigenetics , dna methylation , chromatin immunoprecipitation , histone , biology , methylation , chromatin , imprinting (psychology) , genomic imprinting , epigenome , promoter , locus (genetics) , gene expression , endocrinology , genetics , gene
Background Folate and methyl‐group deficiency has been linked to prostate cancer susceptibility, yet the mechanisms underlying these observations are incompletely understood. The region of the genome containing the imprinted genes insulin‐like growth factor 2 ( Igf2 ) and H19 , both of which display oncogenic functions, may be particularly sensitive to environmental influences. Methods To determine whether a methyl‐deficient diet impacts epigenetic controls at the Igf2 – H19 locus, we placed C57BL/6 mice containing a polymorphism at the imprinted Igf2 – H19 locus on a choline and methionine deficient (CMD) diet. We interrogated this locus for expression and epigenetic changes in prostate tissues. Results A significant increase in both Igf2 and H19 expression was found in CMD prostate tissues compared to controls. These expression changes were reversible with shorter exposure to the CMD diet. Chromatin immunoprecipitation (ChIP) revealed significant decreases in repressive histone modifications (dimethyl‐H3K9) within the H19 promoter, as well as Igf2 P2 and P3 promoters. DNA methylation within these promoters was not altered. No significant change in Igf2 or H19 imprinting was observed. Conclusions These findings highlight the plasticity of the epigenome in an epithelial organ vulnerable to neoplastic change. They further suggest that chromatin modifications are more susceptible to methyl‐deficient diets than DNA methylation at this locus. Prostate 68: 1187–1195, 2008. © 2008 Wiley‐Liss, Inc.