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Increased distributional variance of mitochondrial DNA content associated with prostate cancer cells as compared with normal prostate cells
Author(s) -
Mizumachi Takatsugu,
Muskhelishvili Levan,
Naito Akihiro,
Furusawa Jun,
Fan ChunYang,
Siegel Eric R.,
Kadlubar Fred F.,
Kumar Udaya,
Higuchi Masahiro
Publication year - 2008
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20697
Subject(s) - prostate cancer , lncap , mitochondrial dna , prostate , carcinogenesis , cancer , biology , mitochondrion , cancer cell , cancer research , pathology , medicine , gene , genetics
Background Mitochondria are key organelles for apoptosis, and mitochondrial DNA (mtDNA) content can regulate cancer progression. Increases in mtDNA mutations and deletions have been reported in cancer; however, a detailed investigation of mtDNA content in cancer cells has not yet been conducted. Methods Quantitative real‐time PCR and improved extraction method were established to investigate the mtDNA content in a single prostate cell. Results The heterogeneity of mtDNA content was demonstrated between the clones of prostate cancer cell line LNCaP and individual cells in each clone. To investigate whether large distributional variance of mtDNA content is associated with cancer initiation and/or progression, we first compared PZ‐HPV‐7, an HPV‐transformed normal prostate epithelial cell line, with CA‐HPV‐10, transformed from prostate cancer cells derived from the same donor. We found an enhanced distributional variance of mtDNA content in CA‐HPV‐10. Then, we investigated mtDNA content in individual cells in laser microdisssected cancer and adjacent normal cells from prostate cancer tissue specimens using quantitative real‐time PCR method. Results showed that the mtDNA content per cell follows a higher skewed distribution in cancer cells as compared in normal cells. We also observed that mtDNA content was increased in seven of nine (78%) of prostate cancers compared to normal prostate tissue. Conclusions These results indicate that prostate carcinogenesis may involve dysregulation of mtDNA content. Prostate 68: 408–417, 2008. © 2008 Wiley‐Liss, Inc.