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Prospective diagnostic efficiency of biopsy washing DNA GSTP1 island hypermethylation for detection of adenocarcinoma of the prostate
Author(s) -
Eilers Tyark,
Machtens Stefan,
Tezval Hossein,
Blaue Christoph,
Lichtinghagen Ralf,
Hagemann Jörn,
Jonas Udo,
Serth Jürgen
Publication year - 2007
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20546
Subject(s) - gstp1 , biopsy , medicine , prospective cohort study , dna methylation , malignancy , prostate cancer , pathology , prostate biopsy , receiver operating characteristic , prostate , mesothelioma , adenocarcinoma , cancer , biology , gene , genotype , biochemistry , gene expression
Abstract BACKGROUND The prospective diagnostic efficiency of quantitative glutathione S transferase (GSTP1) promoter hypermethylation analysis in biopsy washing samples has not been determined so far. METHODS Biopsies were obtained prospectively from 86 patients suspicious for prostate cancer (CaP). After isolation of DNA from biopsy washings and bisulfite conversion methylated and unmethylated GSTP1 sequences were specifically quantitated by real‐time fluorescence PCR. Relative degrees of methylation were compared to results of histopathological examination. RESULTS Increased relative methylation was found for the CaP group (mean 28.1%) compared to biopsies without histological evidence for malignancy (5.2%; P < 0.001). A sensitivity and specificity of 92% and 86% and positive and negative predictive values of 82% and 94% were obtained. Receiver operating characteristic (ROC) analysis demonstrated a value of 0.90 (95% CI 0.82–0.98) for the area under curve (AUC). CONCLUSIONS Biopsy washing DNA GSTP1 hypermethylation analysis demonstrates a high diagnostic efficacy which is comparable to the retrospective analysis of biopsy tissue specimens. Moreover it is compatible with routine biopsy examination thus permitting further prospective evaluation in CaP diagnosis. Prostate 67: 757–763, 2007. © 2007 Wiley‐Liss, Inc.