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Gain of chromosome X in prostatic atrophy detected by CGH and FISH analyses
Author(s) -
YildizSezer Seval,
Verdorfer Irmgard,
Schäfer Georg,
Rogatsch Hermann,
Bartsch Georg,
Mikuz Gregor
Publication year - 2007
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20535
Subject(s) - prostate cancer , comparative genomic hybridization , prostate , pathology , atrophy , fluorescence in situ hybridization , cancer , biology , prostatectomy , chromosome , medicine , genetics , gene
BACKGROUND Focal atrophy is presumed to be an indirect forerunner of prostate cancer. The aim of this study was to examine genetic alterations in prostate epithelia deriving from atrophic areas and compare these findings with those of cells deriving from paired prostate cancer in the same patient. METHODS Formalin fixed paraffin wax‐embedded prostatectomy specimens from 20 prostate cancer patients were utilized in this study. Comparative Genomic Hybridization (CGH) was performed on atrophic areas. To validate the CGH results, Fluorescence in Situ Hybridization (FISH) analysis was performed on atrophic areas and paired cancer tissue. RESULTS Gain of the whole chromosome X was found as sole aberration in seven (70%) atrophic tissues by CGH. A gain of centromere X was observed in 13 (68.4%) atrophic areas and in 18 (90%) cancer tissues using FISH. CONCLUSIONS Our investigation reconfirms the genetical instability of cells of the atrophic acini and attention of relevance of gain of chromosome X in atrophic areas. Prostate 67: 433–438, 2007. © 2007 Wiley‐Liss, Inc.