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Quantitative multi‐gene expression profiling of primary prostate cancer
Author(s) -
Schmidt Uta,
Fuessel Susanne,
Koch Rainer,
Baretton Gustavo B.,
Lohse Andrea,
Tomasetti Silke,
Unversucht Susanne,
Froehner Michael,
Wirth Manfred P.,
Meye Axel
Publication year - 2006
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20490
Subject(s) - prostate cancer , pca3 , prostate , medicine , receiver operating characteristic , oncology , prostate specific antigen , housekeeping gene , pathology , cancer research , cancer , biology , gene expression , gene , genetics
BACKGROUND This study describes the evaluation of the expression patterns of prostate‐related transcripts in 106 matched prostate tissues from prostatectomies as predictors for prostate cancer (PCa). METHODS Quantitative PCR (QPCR) assays with site‐specific hybridization probes were established for four housekeeping genes ( GAPDH, HPRT, PBGD, TBP) and nine prostate‐related genes ( AibZIP , D‐GPCR, EZH2, PCA3, PDEF, prostein, PSA, PSCA, TRPM8 ). RESULTS The relative mRNA expression levels of AibZIP, D‐GPCR, EZH2, PCA3, PDEF, PSA, TRPM8 (all P < 0.001) and prostein ( P = 0.019) normalized to the TBP reference gene were significantly higher in malignant compared to non‐malignant prostate tissues. Employing receiver‐operating characteristic (ROC) analyses, PCA3 was the best single tumor marker with the highest area‐under‐the‐curve (AUC = 0.85). A multivariate logit model for the predictability of the tumor was developed, which employed the relative expression levels of EZH2, PCA3, prostein , and TRPM8 and yielded an AUC of 0.90. CONCLUSIONS The transcript marker PCA3 is a powerful predictor of primary PCa but the inclusion of EZH2, prostein , and TRPM8 adds even more to the diagnostic power. The finding of a significantly higher mRNA expression of three different genes ( prostein, PSA, TRPM8 ) in organ‐confined tumors compared to non‐organ‐confined tumors as well as the multi‐marker PCa prediction model developed in the retrospective model system on prostatectomies could be of clinical importance for diagnostic purposes, and should be verified in diagnostic biopsies. Prostate 66: 1521–1534, 2006. © 2006 Wiley‐Liss, Inc.