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The DU145 human prostate carcinoma cell line harbors a temperature‐sensitive allele of p53
Author(s) -
Bajgelman Marcio C.,
Strauss Bryan E.
Publication year - 2006
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20462
Subject(s) - du145 , mutant , allele , cell culture , biology , tumor suppressor gene , cancer research , context (archaeology) , wild type , phenotype , gene , prostate , microbiology and biotechnology , genetics , carcinogenesis , cancer , paleontology , lncap
BACKGROUND Some nuances of mutant and wild‐type p53 activity have been uncovered utilizing temperature sensitive (TS) alleles. However, few human tumor derived cell lines possess a TS p53 mutant. METHODS The cell lines DU145 (heterozygous p53, P223L, and V274F) and PC3 (p53‐null, where exogenous P223L and V274F were introduced individually or in combination) were examined for TS p53 activity as revealed by reporter construct and target gene activation. RESULTS TS p53 function was observed in DU145 and expression of the P223L allele in PC3 conferred a TS p53 profile. Activation of p21Waf1 demonstrated that P223L TS activity may have been influenced by cellular context. CONCLUSIONS The DU145 cell line harbors a TS mutant of p53 and, in addition to being a widely used model of human prostate carcinoma, may also reveal new insights into p53 function due to the unique transcriptional properties of its TS phenotype. Prostate 66: 1455–1462, 2006. © 2006 Wiley‐Liss, Inc.