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Prostate cells express two isoforms of fibroblast growth factor receptor 1 with different affinities for fibroblast growth factor‐2
Author(s) -
Roghani Monireh,
Moscatelli David
Publication year - 2006
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20448
Subject(s) - fibroblast growth factor receptor 1 , fibroblast growth factor , gene isoform , biology , microbiology and biotechnology , fibroblast growth factor receptor , medicine , endocrinology , receptor , fibroblast growth factor receptor 2 , growth factor , heparan sulfate , cancer research , heparin , biochemistry , gene
Background Fibroblast growth factor receptor 1 (FGFR1) mRNA can be alternatively spliced to generate isoforms containing (FGFR1α) or lacking (FGFR1β) the first immunoglobulin‐like domain. We examined which isoforms are expressed by cultured prostate cells, their affinities for FGF‐2, and the effect of heparin on FGF‐2 binding. Methods FGFR1 isoform expression was examined by RT‐PCR. FGFR1α and FGFR1β were expressed in CHO cells mutant in heparan sulfate synthesis, and their affinities for FGF‐2, FGF‐1, FGF‐4, and FGF‐6 were determined in the presence and absence of heparin. Results FGFR1α was expressed in luminal epithelial cells, whereas FGFR1β was expressed in basal epithelial and smooth muscle cells. FGFR1β bound FGF‐2 with three–fourfold higher affinity than FGFR1α both in the presence and absence of heparin. Heparin increased affinity of both receptor isoforms for FGF‐2 approximately four–fivefold. Conclusions Prostate smooth muscle and basal epithelial cells are likely to be more sensitive than luminal epithelial cells to the low concentrations of FGFs present in vivo. Prostate © 2006 Wiley‐Liss, Inc.