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Characterization of the mitochondrial aconitase promoter and the identification of transcription factor binding
Author(s) -
Yu Ziqiang,
Costello Leslie C.,
Feng Pei,
Franklin Renty B.
Publication year - 2006
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20442
Subject(s) - aconitase , transcription factor , biology , mitochondrion , transcription (linguistics) , genetics , microbiology and biotechnology , chemistry , gene , linguistics , philosophy
BACKGROUND Mitochondrial (m) aconitase plays an important role in the unique pathway of citrate accumulation in prostate epithelial cells through its limited activity. In the current study, we characterized the human m‐aconitase gene promoter. METHODS A 1,411‐bp 5′‐flanking fragment of the human m‐aconitase gene was cloned, followed by 5′ serial deletion analysis of promoter activity. Transcriptional start sties and transcription factors bound to the promoter were identified by 5′ RACE, DNA pull‐down assay and transcription factor array analysis. RESULTS Two transcriptional start sites were identified. The promoter fragment pulled down 15 transcription factors, some without consensus sequences in the promoter. Deletion of one Sp1 site eliminated all promoter activity. CONCLUSIONS The m‐aconitase promoter is contained in a 153‐bp 5′ fragment lacking a TATA or CAAT sequence. Sp1 binding to a specific Sp1 site is required for promoter activity while other transcription factors are recruited through protein–protein interactions. Prostate 66: 1061–1069, 2006. © 2006 Wiley‐Liss, Inc.