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Characterization of circulating blood dendritic cell subsets DC123+ (Lymphoid) and DC11C+ (myeloid) in prostate adenocarcinoma patients
Author(s) -
Alessandro Sciarra,
Miriam Lichtner,
AnnaMaria Autran Gomez,
Claudio Mastroianni,
Raffaella Rossi,
Fabio Mengoni,
Cristiano Cristini,
Alessandro Gentilucci,
Vincenzo Vullo,
Franco Di Silverio
Publication year - 2006
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20431
Subject(s) - prostate cancer , prostate , medicine , myeloid , adenocarcinoma , prostate adenocarcinoma , population , stage (stratigraphy) , pathology , oncology , gastroenterology , cancer , biology , paleontology , environmental health
PURPOSE We verified whether prostate adenocarcinoma produces specific modifications in DC subsets count. METHODS Twenty‐one untreated prostate adenocarcinomas were divided on the basis of clinical stage in localized and metastatic disease. As control we used a population of 18 healthy male subjects. For DCs enumeration, peripheral blood (PB) samples were obtained in all cases. A single‐platform flow cytometric assay based on Tru‐COUNT was used for the enumeration of the two DCs subsets, myeloid (mDCs) and plasmacytoid (pDCs). RESULTS We showed a statistically significant reduction in pDCs count in prostate cancer population when compared to healthy controls ( P = 0.002). Comparing each clinical stage with healthy controls, significant differences were found between controls and the metastatic group in both pDCs and mDCs ( P = 0.005 and P = 0.023 respectively) but not between controls and the localized group ( P = 0.055 and P = 0.829 respectively). CONCLUSIONS We showed that DCs count in PB is significantly affected by prostate adenocarcinoma progression in a metastatic disease. Prostate 67:1–7, 2007. © 2006 Wiley‐Liss, Inc.