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Generation of interleukin‐17 receptor‐like protein (IL‐17RL) in prostate by alternative splicing of RNA
Author(s) -
Haudenschild Dominik R.,
Curtiss Shane B.,
Moseley Timothy A.,
Reddi A. Hari
Publication year - 2006
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20422
Subject(s) - alternative splicing , exon , rna splicing , gene isoform , biology , rna , protein isoform , exon skipping , splice , microbiology and biotechnology , messenger rna , oligonucleotide , complementary dna , gene , genetics
BACKGROUND Interleukin‐17 receptor‐like protein (IL‐17RL) expressed in prostate tissues changes with advanced cancers due to extensive alternative splicing, which affects the final protein. Predominant IL‐17RL splice isoform variants have not been identified, hindering functional studies. METHODS A cDNA library of IL‐17RL transcripts was arrayed onto nylon membranes. Individual transcript exon structures were determined by successively probing membranes with exon‐specific oligonucleotides. The most common variants were transiently over‐expressed in 293T cells. RESULTS We detected >90 different IL‐17RL isoforms. Three most abundant isoforms account for approximately half the total transcripts; the full‐length variant just over 11%. Surprisingly, most alternative splicing does not alter the reading frame of the full‐length molecule; therefore, resulting proteins vary mostly in N‐terminal domains. CONCLUSIONS IL‐17RL exists as multiple isoforms due to extensive alternative splicing. We identified the most abundant splices in prostate tissue and established a technique to investigate changes in RNA IL‐17RL splicing that occur in advanced cancers. Prostate © 2006 Wiley‐Liss, Inc.