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Protein tyrosine phosphatase PTP1B is involved in neuroendocrine differentiation of prostate cancer
Author(s) -
Wu Chengyu,
Zhang Li,
Bourne Patricia A.,
Reeder Jay E.,
di Sant'Agnese P. Anthony,
Yao Jorge L.,
Na Yanqun,
Huang Jiaoti
Publication year - 2006
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20412
Subject(s) - lncap , neuroendocrine differentiation , prostate cancer , prostate , biology , cancer research , endocrinology , androgen , medicine , protein tyrosine phosphatase , immunohistochemistry , cancer , tyrosine , hormone , biochemistry
BACKGROUND Prostate cancer (PC) contains a minor component of neuroendocrine (NE) cells that may stimulate androgen‐independent growth of the tumor. The mechanism of neuroendocrine differentiation remains unknown. METHODS The expression of PTP1B, a protein tyrosine phosphatase, was studied in LNCaP cells induced to show neuroendocrine phenotype by androgen withdrawal. Wild‐type PTP1B and its dominant‐negative mutant were transfected into LNCaP cells to study their effects on neuroendocrine differentiation. In vivo expression of PTP1B in human prostate cancer was studied by immunohistochemistry. RESULTS Androgen withdrawal of LNCaP cells led to increased expression of PTP1B with a corresponding increase in its tyrosine phosphatase activity. Overexpression of PTP1B in LNCaP cells led to neuroendocrine differentiation while expression of its dominant‐negative mutant inhibited neuroendocrine differentiation. Immunohistochemical study showed that PTP1B was exclusively expressed in neuroendocrine cells of human prostate cancer tissue. Conclusion Our findings suggest that PTP1B plays an important role in neuroendocrine differentiation, and therefore, may possibly be involved in the progression of prostate cancer. Prostate © 2006 Wiley‐Liss, Inc.