Premium
Regulation of calcium homeostasis by S100RVP, an androgen‐regulated S100 protein in the rat ventral prostate
Author(s) -
Oram Shane,
Cai Xiaoyan,
Haleem Riffat,
Cyriac Jomol,
Wang Zhou
Publication year - 2006
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20395
Subject(s) - calcium , biology , lncap , microbiology and biotechnology , fusion protein , green fluorescent protein , calcium binding protein , transfection , subcellular localization , cytoplasm , medicine , biochemistry , prostate , cancer , gene , genetics , recombinant dna
BACKGROUND S100RVP was previously identified as an androgen‐response gene in the rat ventral prostate (RVP). Characterization of S100RVP is important for elucidating the function of S100 proteins in androgen action. METHODS The expression and subcellular localization of S100RVP were determined by Northern blot, in situ hybridization, and fluorescent microscopy. Calcium overlay and calcium ionophore sensitivity assays were performed to investigate the calcium binding and function of S100RVP. RESULTS S100RVP is abundantly expressed in the RVP epithelial cells. A green fluorescent protein(GFP)‐S100RVP fusion protein is present in both the cytoplasm and nucleus of transfected cells. A GST‐S100RVP fusion protein bound calcium in vitro at levels similar to known S100 proteins. Furthermore, GFP‐S100RVP transfected LNCaP and PC3 cells exhibited reduced sensitivity to calcium ionophore‐induced cell death, but not to UV‐induced cell death. CONCLUSION The results of this study argue for a role of S100RVP in calcium homeostasis in the prostate. Prostate © 2006 Wiley‐Liss, Inc.