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Characterization of an antibody that can detect the Kai1/CD82 murine metastasis suppressor
Author(s) -
Custer Mary C.,
Risinger John I.,
Hoover Shelley,
Simpson R. Mark,
Patterson Tricia,
Barrett J. Carl
Publication year - 2005
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20386
Subject(s) - metastasis suppressor gene , antibody , metastasis suppressor , polyclonal antibodies , tetraspanin , immunohistochemistry , biology , metastasis , cancer research , flow cytometry , antigen , prostate , prostate cancer , pathology , microbiology and biotechnology , immunology , cancer , medicine , cell , genetics
BACKGROUND Kai1, also known as CD82, is a member of the tetraspanin family (TM4SF). The human homolog, KAI1, is an activation antigen of T‐cells and is a metastasis suppressor for prostate and other cancers. Little is known about the mouse protein because of the lack of antibody reagents. METHODS Peptide immunized rabbits were used to generate polyclonal antibody to Kai1. The antibody was analyzed using immunoblotting, flow cytometry, and immunohistochemistry. RESULTS This antibody specifically recognizes murine Kai1 protein, crossreacts with rat Kai1 but not with human KAI1. The normal tissue distribution of this protein in mice is shown to be similar to that of the human homolog. Interestingly, mouse prostatic epithelium showed differential expression within the lobes. CONCLUSION This antibody, the first described that can specifically detect murine Kai1/CD82, should be very useful in addressing the mechanism of action of Kai1 in metastatic suppression. Prostate 66:567–577, 2006. © 2005 Wiley‐Liss, Inc.