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Prostate cell lines secrete IL‐8 in response to mycoplasma hominis through Toll‐like receptor 2‐mediated mechanism
Author(s) -
Takeyama Koh,
Mitsuzawa Hiroaki,
Shimizu Takeyuki,
Konishi Masanori,
Nishitani Chiaki,
Sano Hitomi,
Kunishima Yasuharu,
Matsukawa Masanori,
Takahashi Satoshi,
Shibata Kenichiro,
Tsukamoto Taiji,
Kuroki Yoshio
Publication year - 2005
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20358
Subject(s) - tlr2 , mycoplasma hominis , secretion , cytokine , toll like receptor , biology , cell culture , receptor , microbiology and biotechnology , mycoplasma , immunology , tlr4 , signal transduction , innate immune system , endocrinology , biochemistry , genetics
Background The roles of Toll‐like receptor 2 (TLR2) and Mycoplasma in prostate inflammation remain unclear. We investigated whether Mycoplasma induced inflammatory cytokine secretion through TLR2‐mediated mechanism in prostate cancer cell line. Methods Cell surface expression of TLR2 on PC‐3 cells were examined by flowcytometry. PC‐3 cells were stimulated with Mycoplasma hominis ( M. hominis ), and IL‐8 secretion and NF‐κB activation were examined. Results PC‐3 cells expressed TLR2 mRNA and cell surface TLR2 protein. The membrane fraction of M. hominis induced IL‐8 secretion from PC‐3 cells and NF‐κB activation in a concentration‐dependent manner. Transient transfection of the dominant negative mutant TLR2 P681H into PC‐3 cells attenuated M. hominis ‐induced IL‐8 secretion and NF‐κB activation. Antibody against the extracellular TLR2 domain significantly suppressed M. homnis ‐induced IL‐8 secretion from the prostate cell lines including PC‐3, PrEC, and transformed myofibroblasts. Conclusions These results clearly demonstrate that the prostate cell line can secrete inflammatory cytokine in response to M. hominis through a TLR2‐mediated mechanism. © 2005 Wiley‐Liss, Inc.