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Targeting of pericytes diminishes neovascularization and lymphangiogenesis in prostate cancer
Author(s) -
Ozerdem Ugur
Publication year - 2006
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20346
Subject(s) - tramp , pericyte , lncap , neovascularization , prostate cancer , cancer research , lymphangiogenesis , knockout mouse , pathology , medicine , cancer , angiogenesis , biology , metastasis , endothelial stem cell , receptor , in vitro , biochemistry
BACKGROUND The walls of capillaries in prostate cancer are composed of endothelial cells, and pericytes. NG2 is a transmembrane proteoglycan on nascent pericytes with a functional role in neovascularization. METHODS The anti‐angiogenic effect of hydron pellets containing NG2 neutralizing antibody was quantified in intracorneal PC‐3 and LNCaP xenografts. TRAMP and TRAMP‐C1 tumors grafted in NG2 knockout mice represented intrinsic pericyte targeting. TRAMP and TRAMP‐C1 grafts were analyzed with confocal microscope for microvascular density (MVD) and lymphatic vascular density (LVD). RESULTS NG2 neutralizing antibody decreased corneal neovascularization in PC3 ( P < 0.0001), and LNCaP ( P = 0.0079) xenografts. Mean MVD in TRAMP and TRAMP‐C1 tumors in NG2 knockout mice were 71% ( P = 0.0006) and 63% ( P = 0.0011) lower than wild type controls, respectively . Mean LVD in TRAMP and TRAMP‐C1 tumors in NG2 knockout mice were 73% ( P = 0.0003) and 84% ( P < 0.0001) lower than wild type controls, respectively. CONCLUSIONS Targeting of pericyte‐NG2 decreases neovascularization and lymphangiogenesis in prostate cancer significantly. © 2005 Wiley‐Liss, Inc.