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Cytogenetic and expression profiles associated with transformation to androgen‐resistant prostate cancer
Author(s) -
Pang SeeTong,
Weng WenHui,
FloresMorales Amilcar,
Johansson Björn,
Pourian Mohammad R.,
Nilsson Peter,
Pousette Åke,
Larsson Catharina,
Norstedt Gunnar
Publication year - 2006
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20328
Subject(s) - lncap , prostate cancer , androgen , biology , androgen receptor , cancer research , prostate , dna methylation , cancer , medicine , endocrinology , genetics , gene expression , gene , hormone
BACKGROUND The mechanisms underlying the progression of prostate cancer to androgen‐resistant cancer are still not fully understood. Here, we studied the genetic events associated with this transformation. METHODS The androgen sensitive prostate cancer cells line LNCaP‐FGC and its androgen resistant subline LNCaP‐r were investigated using SKY, CGH, and cDNA microarray. RESULTS Karyotypically, several additional chromosomal aberrations were seen in LNCaP‐r as compared to the parental line. CGH also revealed unique net chromosomal alterations in LNCaP‐r compared to LNCaP‐FGC, including gain of 2p13‐23, 2q21‐32, and 13q and loss of 6p22‐pter. cDNA microarray analysis identified several genes involved in DNA methylation, such as DNMT2 , DNMT3a , and methyl‐CpG binding domain protein 2 and 4 that were higher expressed in LNCaP‐r. Interestingly, androgen responsiveness of LNCaP‐r was restored after treated with DNA methyltransferase inhibitor. CONCLUSIONS Our findings may serve as a basis for molecular dissection of the mechanisms involved in development of androgen resistant prostate cancer. © 2005 Wiley‐Liss, Inc.