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Endogenous and exogenous citrate transport and release in prostatic preparations: semi‐polarized two‐dimensional cultures of human PNT2‐C2 cells and isolated tubules and segments of rat prostate
Author(s) -
Mycielska Maria E.,
Krasowska Monika,
Grzywna Zbigniew,
Djamgoz Mustafa B.A.
Publication year - 2005
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20274
Subject(s) - endogeny , extracellular , prostate , intracellular , cell culture , chemistry , monolayer , biophysics , endocrinology , biology , biochemistry , medicine , cancer , genetics
BACKGROUND Electrophysiological characterization of normal human prostate epithelial cells showed exogenous trivalent citrate transport (release) to be K + ‐dependent. METHODS (1) Ussing chamber recordings of short circuit current (SCC) were used to study citrate transport in the same (PNT2‐C2) cell line grown on micro‐pore filters as a monolayer. (2) Release of endogenous citrate from confluent cultures and tubules and segments of rat prostate was measured using a fluorescence technique. (3) Enzyme‐spectrophotometry was employed to detect citrate release from segments of rat prostate. RESULTS Citrate transport across the PNT2‐C2 monolayer was asymmetrical, consistent with release into the lumen‐side. Fluorescence and/or enzyme‐spectrophotometric measurements showed that time‐dependent citrate release (endogeneous and preabsorbed) occurred from rat prostate (tubules and segments), but not kidney or lung. The release was dependent on extracellular K + but not Na + . CONCLUSIONS Citrate release from prostatic cells and tissues (rat and human) was K + ‐dependent, consistent with the previous electrophysiological data. © 2005 Wiley‐Liss, Inc.