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Bi‐directional interactions of prostate cancer cells and bone marrow endothelial cells in three‐dimensional culture
Author(s) -
Barrett Jeffrey M.,
Mangold Kathy A.,
Jilling Tamas,
Kaul Karen L.
Publication year - 2005
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20206
Subject(s) - lncap , bone marrow , prostate cancer , cancer cell , angiogenesis , cancer research , cell culture , cell growth , pathology , medicine , biology , cancer , microbiology and biotechnology , chemistry , biochemistry , genetics
Abstract BACKGROUND Prostate cancer preferentially metastasizes to bone, yet little is known about the cellular and molecular factors that support this growth. Endothelial cells are likely the initial contact for circulating prostate cells entering the bone microenvironment. METHODS Using co‐culture and conditioned media experiments, we studied cellular and molecular interactions of prostate cancer cells of varying aggressiveness (PC‐3 and LNCaP) with bone marrow endothelial (HBME‐1) cells in collagen gels. RESULTS In co‐culture, HBME‐1 cells stimulated proliferation (∼90% increase) and migration of the more aggressive PC‐3 cell line, while having little effect on LNCaP cell proliferation or migration. Concomitantly, HBME‐1 cell growth was inhibited by both PC‐3 and LNCaP cells and their conditioned media. Additionally, HBME‐1 cells underwent significant morphological changes in co‐culture, forming large, branching, cord‐like structures, which mimic angiogenesis. Prostate cancer cell conditioned media induced a similar effect on HBME‐1 cells. In comparison, conditioned media from PC‐3 cells also inhibited growth of non‐bone marrow‐derived endothelial cells, but did not affect their morphology. CONCLUSIONS Significant bi‐directional interactions, including secreted factors and direct cellular interactions, exist between bone marrow endothelial cells and highly metastatic prostate cancer cells, and may underlie the propensity for prostate cancer to metastasize to the bone. © 2005 Wiley‐Liss, Inc.