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Characterization of a lymph node within the mouse prostate: Detailed analysis using whole mount histology
Author(s) -
Gerber Scott A.,
Turner Michael J.,
Lugade Amit A.,
Moran James P.,
Frelinger John G.,
Lord Edith M.
Publication year - 2005
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20169
Subject(s) - prostate , lymph node , lymphatic system , high endothelial venules , pathology , histology , lymph , prostate cancer , medicine , immune system , immunohistochemistry , biology , anatomy , immunology , cancer
Abstract BACKGROUND Due to the prevalence of prostate disease, there is a growing interest in the immunobiology of the prostate and its contribution to such pathologies. Further study is needed to fully characterize immune responses within the prostate. METHODS Mouse ventral prostates were removed and analyzed using conventional immunohistochemistry, or by a novel whole mount technique, which allows the visualization of complete structures within the prostate. RESULTS A lymphoid structure was detected within the base of the mouse ventral prostate. Whole mount and cryostat sections revealed an organized arrangement of lymphocytes, along with dendritic cells interspersed throughout the node. It possessed a rich network of lymph node specific high endothelial venules (HEVs) and adjoining lymphatics containing immune cells. CONCLUSIONS Our results demonstrate the presence of a lymphoid structure within the base of the mouse ventral prostate possessing traits characteristic of an organized peripheral lymph node. © 2004 Wiley‐Liss, Inc.