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Genome‐wide characterization of gene expression variations and DNA copy number changes in prostate cancer cell lines
Author(s) -
Zhao Hongjuan,
Kim Young,
Wang Pei,
Lapointe Jacques,
Tibshirani Rob,
Pollack Jonathan R.,
Brooks James D.
Publication year - 2005
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20158
Subject(s) - copy number variation , biology , gene , dna microarray , prostate cancer , chromoplexy , copy number analysis , gene expression , human genome , context (archaeology) , dna , gene dosage , genetics , gene expression profiling , genome , cancer , cancer research , pca3 , paleontology
BACKGROUND The aim of this study was to characterize gene expression and DNA copy number profiles in androgen sensitive (AS) and androgen insensitive (AI) prostate cancer cell lines on a genome‐wide scale. METHODS Gene expression profiles and DNA copy number changes were examined using DNA microarrays in eight commonly used prostate cancer cell lines. Chromosomal regions with DNA copy number changes were identified using cluster along chromosome (CLAC). RESULTS There were discrete differences in gene expression patterns between AS and AI cells that were not limited to androgen‐responsive genes. AI cells displayed more DNA copy number changes, especially amplifications, than AS cells. The gene expression profiles of cell lines showed limited similarities to prostate tumors harvested at surgery. CONCLUSIONS AS and AI cell lines are different in their transcriptional programs and degree of DNA copy number alterations. This dataset provides a context for the use of prostate cancer cell lines as models for clinical cancers. © 2004 Wiley‐Liss, Inc.