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Characterization of prostatic neuroendocrine cell line established from neuroendocrine carcinoma of transgenic mouse allograft model
Author(s) -
Uchida Kohsuke,
Masumori Naoya,
Takahashi Atsushi,
Itoh Naoki,
Tsukamoto Taiji
Publication year - 2005
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20111
Subject(s) - cell culture , neuroendocrine differentiation , immortalised cell line , carcinogenesis , prostate , androgen receptor , cancer research , biology , prostate cancer , somatostatin , cell growth , in vitro , pathology , medicine , endocrinology , cancer , genetics
BACKGROUND The role of neuroendocrine (NE) cells in prostate cancer remains unclear. A useful model is necessary to study the biology of NE cells. We herein describe the establishment and characterization of an immortalized cell line from an NE‐10 allograft of murine prostatic NE carcinoma. METHODS A novel cell line, designated NE‐CS, was developed from an NE‐10 allograft that was established from the ventral prostate of the LPB‐T‐antigen (Tag) transgenic mouse, line 10 (12T‐10). We investigated the growth, karyotype, electron microscopic findings, expression of Tag and androgen receptor (AR), and tumorigenesis of the cells in athymic mice. RESULTS The immortal cell line NE‐CS was maintained in vitro for more than 2 years. The NE‐CS cells had dendritic‐like extensions with dense core granules in the cytoplasm and produced serotonin and somatostatin in conditioned medium. The cells expressed neither Tag nor AR. They showed androgen‐independent growth in vitro and a hypotetraploid karyotype similar to the original NE‐10 allograft. The NE‐CS cells, which were subcutaneously inoculated into athymic mice, formed tumors with the NE phenotype. The tumors exhibited accelerated growth compared to the original NE‐10 allograft. CONCLUSIONS The established cell line has characteristics of NE differentiation and tumorigenic ability. This cell line may be a promising model to understand the molecular mechanisms associated with the acquisition of hormone refractory prostate cancer. © 2004 Wiley‐Liss, Inc.