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Transfer of functional prostasomal CD59 of metastatic prostatic cancer cell origin protects cells against complement attack
Author(s) -
Babiker Adil A.,
Nilsson Bo,
Ronquist Gunnar,
Carlsson Lena,
Ekdahl Kristiilsson
Publication year - 2004
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.20102
Subject(s) - lncap , du145 , cd59 , prostate cancer , cancer research , cancer cell , cell , complement system , prostate , biology , chemistry , cancer , antibody , immunology , medicine , biochemistry
BACKGROUND Prostasomes are secretory granules produced, stored, and released, by the glandular epithelial cells of the prostate. They express the glycosylphosphatidylinositol (GPI)‐anchored complement regulatory protein CD59, which has been shown to be transferred to spermatozoa and erythrocytes. METHODS The CD59 content of prostasomes isolated from seminal fluid and malignant prostate cells (PC‐3, DU145, and LNCaP) and the transfer of prostasomal CD59 to rabbit erythrocytes (RE) and to PIPLC‐treated and unmanipulated cancer cells were investigated using FACS. All prostasomes were also incubated with RE and tested in a hemolytic assay. RESULTS Prostasomes from cancer cells had higher expression of CD59 than those of normal cells. Prostasomal CD59 of different origin could be transferred to RE, malignant cell lines stripped of CD59 by PIPLC, or unmanipulated LNCaP cells. Malignant cell prostasomes had an increased ability to inhibit complement‐mediated lysis compared to those from non‐malignant cells. CONCLUSIONS These results point to a novel mechanism by which prostasomes can protect prostatic malignant cells from complement attack. © 2004 Wiley‐Liss, Inc.