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Metabolic susceptibility genes and prostate cancer risk in a southern European population: The role of glutathione S‐transferases GSTM1, GSTM3, and GSTT1 genetic polymorphisms
Author(s) -
Medeiros Rui,
Vasconcelos André,
Costa Sandra,
Pinto Daniela,
Ferreira Paula,
Lobo Francisco,
Morais António,
Oliveira Jorge,
Lopes Carlos
Publication year - 2003
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.10348
Subject(s) - genotype , prostate cancer , allele , odds ratio , medicine , biology , glutathione , confidence interval , genetics , case control study , gstp1 , cancer , oncology , glutathione s transferase , gastroenterology , gene , enzyme , biochemistry
Abstract BACKGROUND Glutathione S‐transferase (GST) metabolic enzymes may be involved in the development of human cancer. Genetic polymorphisms have been reported in GSTM1, GSTM3, and GSTT1 with functional alterations and influencing cancer risk. METHODS We analyzed DNA samples from 335 (670 alleles) unrelated individuals, 185 community control subjects, and 150 prostate cancer (PC) patients, for GSTM1, GSTM3, and GSTT1 genotypes using polymerase chain reaction (PCR). RESULTS The analysis of the frequencies from the 670 alleles indicates that men carrying two B‐alleles (GSTM3) have increased risk for PC (OR = 5.50, 95% confidence interval (CI) 1.2–25.8; P = 0.016). Multivariate logistic regression analysis confirmed this association (OR = 5.2, 95% CI 1.1–25.0; P = 0.036). No increased PC risk was observed for men carrying any of the GSTM1 or GSTT1 genotypes (OR = 1.20, 95% CI 0.75–1.90; P = 0.420 for GSTM1 null and OR = 0.87, 95% CI 0.50–1.51; P = 0.550 for GSTM1 null). However, GSTT1 null was overrepresented in men with advanced PC disease ( P = 0.038). CONCLUSIONS Our results indicate that polymorphism in the GSTM3 may be an important biomarker for PC risk, especially in the definition of the genetic risk profile of populations of southern Europe. © 2003 Wiley‐Liss, Inc.