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Membrane properties of single muscle cells of the rhabdosphincter of the male urethra
Author(s) -
Berjukow Stanislav,
Margreiter Eva,
Marksteiner Rainer,
Strasser Hannes,
Bartsch Georg,
Hering Steffen
Publication year - 2004
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.10334
Subject(s) - urethra , male urethra , urology , anatomy , medicine
BACKGROUND The electrophysiological properties of myoblast cultures established from the human and porcine rhabdosphincter (RS) and porcine lower limb muscle (LLSKM) were studied to elucidate their potential for tissue engineering applications in the lower urinary tract. METHODS Muscle biopsies were collected from the prostatic part of the RS, the RS of male pigs, and the porcine LLSKM. Ion channels were studied by means of the patch‐clamp technique. RESULTS Only one subtype each of voltage gated Na + and Ca 2+ channels was observed in porcine RS and LLSKM. Two types of voltage gated Ca 2+ channels were identified in human RS cells. The porcine RS and LLSKM myoblasts displayed similar fusion competence. CONCLUSIONS Porcine RS and LLSKM myoblasts and human RS and human skeletal muscle cells show a high degree of similarity. Injection of autologous skeletal muscle myoboblasts in the lower urinary tract might, therefore, represent a promising approach to treat stress incontinence after radical prostatectomy. © 2004 Wiley‐Liss, Inc.