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Expression of ERα and ERβ in prostate cancer
Author(s) -
Linja Marika J.,
Savinainen Kimmo J.,
Tammela Teuvo L.J.,
Isola Jorma J.,
Visakorpi Tapio
Publication year - 2003
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.10242
Subject(s) - du145 , prostate cancer , prostate , lncap , hyperplasia , androgen receptor , medicine , pca3 , immunohistochemistry , estrogen receptor , androgen , cancer research , cancer , hormone , breast cancer
BACKGROUND It has been suggested that estrogens and their receptors (ERs) may be involved in the development and progression of prostate cancer. To elucidate the significance of these receptors, expression of both ERα and ERβ was measured in benign and malignant prostate tumors, as well as in cell lines. METHODS Expression of ERα and ERβ was measured in prostate hyperplasia (BPH, n = 7), androgen‐dependent (n = 30) as well as hormone‐refractory (n = 12) prostate carcinomas, and in four prostate cancer cell lines (LNCaP, DU145, PC‐3, and 22Rv1) using real‐time quantitative RT‐PCR. RESULTS Only low‐level expression of ERα was found in all tumor types and cell lines. The level of expression was similar to that observed in breast carcinomas found to be negative for ERα by immunohistochemistry. All cell lines showed low, but detectable, levels of ERβ expression. The mean expression of ERβ in the hormone‐refractory carcinomas was about half that seen in BPH or the androgen‐dependent carcinomas; however, the difference was not statistically significant. CONCLUSIONS The data suggest it is unlikely that alterations in the expression of either ER are commonly involved in the progression of prostate cancer. Prostate 55: 180–186, 2003. © 2003 Wiley‐Liss, Inc.