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Characteristics of human prostasomes isolated from three different sources
Author(s) -
Carlsson Lena,
Nilsson Ove,
Larsson Anders,
Stridsberg Mats,
Sahlén Göran,
Ronquist Gunnar
Publication year - 2003
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.10189
Subject(s) - semen , bone metastasis , biology , prostate , chromogranin a , metastasis , flow cytometry , endocrinology , cancer , microbiology and biotechnology , immunology , anatomy , immunohistochemistry , genetics
Abstract Background Prostasomes are organelles secreted by human prostate epithelial cells and appear freely in semen. Most prostasomal functional and biochemical characteristics have been settled on basis of seminal prostasomes (SP). Methods Three types of prostasomes, viz . , from seminal plasma, prostate gland (native), and prostate cancer bone metastasis, were isolated and compared for their biochemical and functional characteristics. Results Flow cytometry showed that all three types of prostasomes had a similar size distribution and electrophoresis revealed close similarities in banding pattern. Also, a high cholesterol/phospholipid ratio and some marker enzymes were distinctive common features. The unusual chromogranin B (CgB)/chromogranin A (CgA) ratio with CgB in abundance over CgA, previously found only in SP, was also valid for the native (NP) and metastasis‐derived prostasomes (MP). The latter prostasome type contained a 10‐fold higher amount of CgA compared to the others. SP and MP showed the same promotive effect on sperm forward motility, whereas NP did not. All three prostasome types exhibited an antibacterial activity against Bacillus megaterium . Conclusions Since the three prostasome types were isolated from different environments, our data support the view that the common characteristics were prostasome‐genuine. Prostate 54: 322–330, 2003. © 2003 Wiley‐Liss, Inc.