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Differentiation and stromal‐induced growth promotion of murine prostatic tumors
Author(s) -
Salm Sarah N.,
Takao Tetsuya,
Tsujimura Akira,
Coetzee Sandra,
Moscatelli David,
Wilson E. Lynette
Publication year - 2002
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.10075
Subject(s) - stromal cell , basal (medicine) , cell culture , biology , pathology , epithelium , cancer research , stroma , endocrinology , medicine , immunohistochemistry , insulin , genetics
BACKGROUND We have derived a panel of p53‐null prostatic “basal” and “luminal” epithelial cell lines and their ras transformed counterparts to study stromal/epithelial interactions and the properties of tumors arising from “basal” and “luminal” cells. METHODS Previously derived normal murine prostatic “basal” epithelial (PE‐B‐1) and “luminal” epithelial (PE‐L‐1) cell lines were transformed with N‐Ras. These lines and a spontaneously transformed “luminal” cell line were inoculated subcutaneously or orthotopically into athymic mice, alone or in combination with normal prostatic smooth muscle cells (SMC). RESULTS All transformed lines formed subcutaneous tumors. SMC significantly enhanced the growth rate of the tumors arising from the “basal” and one of the “luminal” cell lines. The transformed “basal” line gave rise to tumors expressing both “basal” and “luminal” cytokeratins. CONCLUSIONS Prostatic SMC promote the growth of transformed epithelial cells, suggesting that prostatic stroma may promote tumor development. Furthermore, transformed “basal” cells give rise to tumors containing “luminal” cells, suggesting that although most human tumors have a “luminal” phenotype, they may originate from transformed “basal” cells. Prostate 51: 175–188, 2002. © 2002 Wiley‐Liss, Inc.