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Human prostate cancer cell death by novel anticancer compounds, apoptosis‐inducing nucleosides from CD57 + HLA‐DR bright natural suppressor cell line
Author(s) -
Guo Maowu,
Sato Eimei,
Jin Aishun,
Li Xiang,
Mori Etsuko,
Xu Yong,
Mori Tsuneatsu
Publication year - 2002
Publication title -
the prostate
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.295
H-Index - 123
eISSN - 1097-0045
pISSN - 0270-4137
DOI - 10.1002/pros.10043
Subject(s) - apoptosis , dna fragmentation , flow cytometry , cell culture , tunel assay , programmed cell death , microbiology and biotechnology , biology , fragmentation (computing) , poly adp ribose polymerase , cancer cell , cell , dna , chemistry , biochemistry , cancer , genetics , polymerase , ecology
BACKGROUND We validated the induction of apoptosis in human prostate cancer PC3 cells by apoptosis‐inducing nucleosides (AINs) released from the CD57 + HLA‐DR bright ‐natural suppressor (57.DR‐NS) cell line. We analyzed the molecular signaling pathway during AINs‐induced apoptosis in PC3 cells. METHODS Direct and indirect co‐cultures between 57.DR‐NS and PC3 cells were performed. AINs were isolated by high‐performance liquid chromatography (HPLC) from 57.DR‐NS cell cultures. Apoptosis in PC3 cells was analyzed by DNA fragmentation, sub‐G 1 DNA content with flow cytometry, and terminal deoxynucleotide transferase–mediated dUTP nick end‐labeling (TUNEL) method. The DNA strand breaks and activation of caspase‐3 in PC3 cells were measured by DNA unwinding and flow cytometry assay. RESULTS The 57.DR‐NS cell line generated apoptosis in PC3 cells via AINs. AINs isolated from 57.DR‐NS cell cultures induced apoptosis in PC3 cells. Furthermore, we found DNA strand breaks followed by activation of caspase‐3 during AINs‐induced apoptosis in PC3 cells. CONCLUSIONS The data obtained here indicated that AINs could induce apoptosis in PC3 cells through DNA strand breaks and activation of caspase‐3. Prostate 51: 166–174, 2002. © 2002 Wiley‐Liss, Inc.