Premium
A fluorescent fatty acid probe, DAUDA, selectively displaces two myristates bound in human serum albumin
Author(s) -
Wang Yu,
Luo Zhipu,
Shi Xiaoli,
Wang Huan,
Nie Lei,
Huang Mingdong
Publication year - 2011
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1002/pro.749
Subject(s) - human serum albumin , chemistry , binding site , fluorophore , fluorescence , albumin , fatty acid , serum albumin , biochemistry , stereochemistry , physics , quantum mechanics
11‐(Dansylamino) undecanoic acid (DAUDA) is a dansyl‐type fluorophore and has widely used as a probe to determine the binding site for human serum albumin (HSA). Here, we reported that structure of HSA‐Myristate‐DAUDA ternary complex and identified clearly the presence of two DAUDA molecules at fatty acid (FA) binding site 6 and 7 of HSA, thus showing these two sites are weak FA binding sites. This result also show that DAUDA is an appropriate probe for FA site 6 and 7 on HSA as previous studied, but not a good probe of FA binding site 1 that is likely bilirubin binding site on HSA.