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Green fluorescent protein: A perspective
Author(s) -
Remington S. James
Publication year - 2011
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1002/pro.684
Subject(s) - green fluorescent protein , fluorescence , fluorescent protein , bimolecular fluorescence complementation , biophysics , chemistry , protein function , biology , biochemistry , physics , gene , quantum mechanics
A brief personal perspective is provided for green fluorescent protein (GFP), covering the period 1994–2011. The topics discussed are primarily those in which my research group has made a contribution and include structure and function of the GFP polypeptide, the mechanism of fluorescence emission, excited state protein transfer, the design of ratiometric fluorescent protein biosensors and an overview of the fluorescent proteins derived from coral reef animals. Structure‐function relationships in photoswitchable fluorescent proteins and nonfluorescent chromoproteins are also briefly covered.