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Monitoring metal ion flux in reactions of metallothionein and drug‐modified metallothionein by electrospray mass spectrometry
Author(s) -
Zaia Joseph,
Fabris Daniele,
Wei Dong,
Karpel Richard L.,
Fenselau Catherine
Publication year - 1998
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1002/pro.5560071117
Subject(s) - chemistry , metallothionein , metalloprotein , mass spectrometry , electrospray ionization , metal ions in aqueous solution , metal , electrospray , zinc , inorganic chemistry , chromatography , organic chemistry
Abstract The capabilities of electrospray ionization mass spectrometry are demonstrated for monitoring the flux of metal ions out of and into the metalloprotein rabbit liver metallothionein and, in one example, chlorambucil‐alkylated metallothionein. Metal ion transfers may be followed as the reactions proceed in situ to provide kinetic information. More uniquely to this technique, metal ion stoichiometries may be determined for reaction intermediates and products. Partners used in these studies include EDTA, carbonic anhydrase, a zinc‐bound hexamer of insulin, and the core domain of bacteriophage T4 gene 32 protein, a binding protein for single‐stranded DNA.