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Identification of the binding site on S100B protein for the actin capping protein CapZ
Author(s) -
Kilby Peter M.,
Roberts GORDON C. K.,
Van Eldik Linda J.
Publication year - 1997
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1002/pro.5560061202
Subject(s) - binding site , chemistry , calcium binding protein , dimer , protein structure , protein–protein interaction , biophysics , plasma protein binding , a site , protein secondary structure , helix (gastropod) , peptide , biochemistry , calcium , biology , ecology , organic chemistry , snail
The calcium‐binding protein S100B binds to several potential target proteins, but there is no detailed information showing the location of the binding site for any target protein on S100B. We have made backbone assignments of the calcium‐bound form of S100B and used chemical‐shift changes in spectra of 15 N‐labeled protein to locate the site that binds a peptide corresponding to residues 265‐276 from CapZα, the actin capping protein. The largest chemical‐shift changes are observed for resonances arising from residues around the C terminus of the C‐terminal helix of S100B and residues Val‐8 to Asp‐12 of the N‐terminal helix. These residues are close to but not identical to residues that have been identified by mutational analysis to be important in other S100 protein‐protein interactions. They make up a patch across the S100B dimer interface and include some residues that are quite buried in the structure of calcium‐free S100B. We believe we may have identified a binding site that could be common to many S100 protein—protein interactions.