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Ala‐insertion scanning mutagenesis of the glycophorin a transmembrane helix: A rapid way to map helix‐helix interactions in integral membrane proteins
Author(s) -
Mingarro Ismael,
Whitley Paul,
Heijne Gunnar Von,
Lemmon Mark A.
Publication year - 1996
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1002/pro.5560050712
Subject(s) - glycophorin , transmembrane domain , helix (gastropod) , mutagenesis , transmembrane protein , alanine scanning , membrane protein , integral membrane protein , saturated mutagenesis , chemistry , biophysics , biology , biochemistry , membrane , mutation , mutant , gene , ecology , receptor , snail
Alanine insertions into the glycophorin A transmembrane helix are found to disrupt helix‐helix dimerization in a way that is fully consistent with earlier saturation mutagenesis data, suggesting that Ala‐insertion scanning can be used to rapidly map the approximate location of structurally and/or functionally important segments in transmembrane helices.