Ala‐insertion scanning mutagenesis of the glycophorin a transmembrane helix: A rapid way to map helix‐helix interactions in integral membrane proteins | Zendy

Mingarro Ismael | Zendy; Whitley Paul | Zendy; Heijne Gunnar Von | Zendy; Lemmon Mark A. | Zendy

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Ala‐insertion scanning mutagenesis of the glycophorin a transmembrane helix: A rapid way to map helix‐helix interactions in integral membrane proteins

Author(s) -

Mingarro Ismael,

Whitley Paul,

Heijne Gunnar Von,

Lemmon Mark A.

Publication year - 1996

Publication title -

protein science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.353

H-Index - 175

eISSN - 1469-896X

pISSN - 0961-8368

DOI - 10.1002/pro.5560050712

Subject(s) - glycophorin , transmembrane domain , helix (gastropod) , mutagenesis , transmembrane protein , alanine scanning , membrane protein , integral membrane protein , saturated mutagenesis , chemistry , biophysics , biology , biochemistry , membrane , mutation , mutant , gene , ecology , receptor , snail

Alanine insertions into the glycophorin A transmembrane helix are found to disrupt helix‐helix dimerization in a way that is fully consistent with earlier saturation mutagenesis data, suggesting that Ala‐insertion scanning can be used to rapidly map the approximate location of structurally and/or functionally important segments in transmembrane helices.

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