Premium
Crystallization of the Aα subunit of protein phosphatase 2A
Author(s) -
Skidmore Michael O.,
Walter Gernot,
Sawaya Michael R.,
Everse Stephen J.,
Parkin Sean,
Rupp Bernhard,
Hope Håkon
Publication year - 1996
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1002/pro.5560050625
Subject(s) - crystallization , protein subunit , phosphatase , chemistry , protein phosphatase 2 , biophysics , protein crystallization , biochemistry , crystallography , biology , enzyme , organic chemistry , gene
The Aα subunit of human protein phosphatase 2A forms crystals in space group P 2 1 with cell dimensions a = 104.0, b = 174.9, c = 168.2 Å, and β angle = 90.2°. At cryogenic temperatures, the crystals diffracted to a resolution limit of ∼ 3.0 Å. Based on the unit cell dimensions and a calculated molecular mass of 65,277 Da, the Matthews coefficient suggests eight molecules per asymmetric unit. Two native data sets were collected to a nominal resolution of 3.0 Å and merged to provide a set that is 93% complete, with R svm of 9.9%.