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Direct evidence for a two‐state protein unfolding transition from hydrogen‐deuterium exchange, mass spectrometry, and NMR
Author(s) -
Yi Qian,
Baker David
Publication year - 1996
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1002/pro.5560050608
Subject(s) - chemistry , deuterium , hydrogen–deuterium exchange , population , mass spectrometry , excited state , nmr spectra database , molecule , proton , analytical chemistry (journal) , crystallography , spectral line , atomic physics , chromatography , organic chemistry , nuclear physics , physics , demography , astronomy , sociology
We use mass spectrometry in conjunction with hydrogen‐deuterium exchange and NMR to characterize the conformational dynamics of the 62‐residue IgG binding domain of protein L under conditions in which the native state is marginally stable. Mass spectra of protein L after short incubations in D 2 O reveal the presence of two distinct populations containing different numbers of protected protons. NMR experiments indicate that protons in the hydrophobic core are protected in one population, whereas all protons are exchanged for deuterons in the other. As the exchange period is increased, molecules are transferred from the former population to the latter. The absence of molecules with a subset of the core protons protected suggests that exchange occurs in part via a highly concerted transition to an excited state in which all protons exchange rapidly with deuterons. A steady increase in the molecular weight of the population with protected protons, and variation in the exchange rates of the individual protected protons indicates the presence of an additional exchange mechanism. A simple model in which exchange results from rapid (>10 5 /s) local fluctuations around the native state superimposed upon transitions to an unfolded excited state at ∼0.06/s is supported by qualitative agreement between the observed mass spectra and the mass spectra simulated according to the model using NMR‐derived estimates of the proton exchange rates.