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Crystallization and preliminary X‐ray investigation of lipoxygenase‐3 from soybeans
Author(s) -
Axelrod Bernard,
Steczko Janusz,
Minor Wladek,
Stojanoff Vivian
Publication year - 1995
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1002/pro.5560040620
Subject(s) - monoclinic crystal system , crystallization , polyethylene glycol , crystallography , lipoxygenase , molecule , chemistry , diffusion , analytical chemistry (journal) , crystal structure , chromatography , enzyme , organic chemistry , thermodynamics , physics
Soybean lipoxygenase‐3 has been crystallized by the vapor diffusion method in 16–20% polyethylene glycol (average M r , 3,400), 0.2 M sodium acetate buffer, pH 5.7, at 21 °C, at a protein concentration of 8–15 mg/mL. The crystals, which diffract to 3‐Å spacings, belong to the monoclinic space group C2. Cell constants are a = 111.9, b= 136.4, and c = 61.6 Å and β = 95.7°. The calculated value of Matthews's constant, V m = 2.48 Å 3 /kDa, is consistent with the presence of one molecule of lipoxygenase per crystallographic asymmetric unit (Z = 4).