Crystallization and preliminary X‐ray investigation of lipoxygenase‐3 from soybeans

Soybean lipoxygenase‐3 has been crystallized by the vapor diffusion method in 16–20% polyethylene glycol (average M r , 3,400), 0.2 M sodium acetate buffer, pH 5.7, at 21 °C, at a protein concentration of 8–15 mg/mL. The crystals, which diffract to 3‐Å spacings, belong to the monoclinic space group C2. Cell constants are a = 111.9, b= 136.4, and c = 61.6 Å and β = 95.7°. The calculated value of Matthews's constant, V m = 2.48 Å 3 /kDa, is consistent with the presence of one molecule of lipoxygenase per crystallographic asymmetric unit (Z = 4).