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Determining protein loop conformation using scaling‐relaxation techniques
Author(s) -
Zheng Qiang,
Rosenfeld Rakefet,
Vajda Sandor,
Delisi Charles
Publication year - 1993
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1002/pro.5560020806
Subject(s) - loop (graph theory) , scaling , relaxation (psychology) , loop modeling , extension (predicate logic) , algorithm , boundary (topology) , protein structure , physics , computer science , chemistry , crystallography , biological system , mathematics , protein structure prediction , geometry , combinatorics , nuclear magnetic resonance , biology , mathematical analysis , neuroscience , programming language
We recently developed a rapid loop closure algorithm in which bond lengths are scaled to constrain the ends of a segment to match a known distance and then gradually relaxed to their standard values, with boundary constraints maintained. Although the algorithm predicted the Zif286 zinc‐finger loop to within approximately 2 Å, it had a serious limitation that made its more general use tentative: it omitted the atomic environment of the loop. Here we report an extension of the algorithm to take into account the protein environment surrounding a given loop from the outset of the conformational search and show that it predicts structure with an efficiency and accuracy that could not be achieved without continuous environmental inclusion. The algorithm should be widely applicable to structure determination when complete experimental information is unavailable.