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Comparison of the X‐ray structure of native rubredoxin from pyrococcus furiosus with the NMR structure of the zinc‐substituted protein
Author(s) -
Blake Paul R.,
Day Michael W.,
Hsu Barbara T.,
JoshuaTor Leemor,
Park JaeBum,
Hare Dennis R.,
Adams Michael W. W.,
Rees Douglas C.,
Summers Michael F.
Publication year - 1992
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1002/pro.5560011113
Subject(s) - rubredoxin , pyrococcus furiosus , crystallography , zinc , chemistry , protein structure , zinc finger , biochemistry , organic chemistry , archaea , gene , transcription factor
The three‐dimensional X‐ray structures of the oxidized and reduced forms of rubredoxin from Pyrococcus furiosus , determined at ‐161°C, and the NMR structure of the zinc‐substituted protein, determined in solution at 45°C, are compared. The NMR and X‐ray structures, which were determined independently, are very similar and lead to similar conclusions regarding the interactions that confer hyperthermostability.