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The disulfide bond pairing of the pheromones E r ‐1 and E r ‐2 of the ciliated protozoan Euplotes raikovi
Author(s) -
Stewart Albert E.,
Raffioni Simona,
Bradshaw Ralph A.,
Chaudhary Tanuja,
Chait Brian T.,
Luporini Pierangelo
Publication year - 1992
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1002/pro.5560010609
Subject(s) - sex pheromone , chemistry , disulfide bond , pairing , cystine , stereochemistry , cysteine , molecule , biochemistry , enzyme , biology , organic chemistry , genetics , physics , superconductivity , quantum mechanics
The disulfide pairings of the two Euplotes raikovi pheromones E r ‐1 and E r ‐2 have been determined by chemical and mass spectrometric analyses. Cystine‐linked peptides from thermolytic digestions of the native molecules were purified by reverse‐phase high performance liquid chromatography and identified in the known sequences to make the assignments. The same pairing, Cys(I)–Cys(IV), Cys(II)–Cys(VI), and Cys(III)–Cys(V), was found in both pheromones, suggesting that this pattern occurs commonly throughout this family of molecules. This arrangement of disulfides indicates that the three‐dimensional structure is defined by three loops, which can vary in size and charge distribution from one pheromone to another.