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Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut
Author(s) -
BastiaanNet Shanna,
PinaPérez Maria C.,
Dekkers Bas J. W.,
Westphal Adrie H.,
America Antoine H. P.,
Ariëns Renata M. C.,
Jong Nicolette W.,
Wichers Harry J.,
Mes Jurriaan J.
Publication year - 2020
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1002/pro.3856
Subject(s) - in silico , cashew nut , chemistry , gene , biology , food science , biochemistry
Proteins from cashew nut can elicit mild to severe allergic reactions. Three allergenic proteins have already been identified, and it is expected that additional allergens are present in cashew nut. pathogenesis‐related protein 10 (PR10) allergens from pollen have been found to elicit similar allergic reactions as those from nuts and seeds. Therefore, we investigated the presence of PR10 genes in cashew nut. Using RNA‐seq analysis, we were able to identify several PR10‐like transcripts in cashew nut and clone six putative PR10 genes. In addition, PR10 protein expression in raw cashew nuts was confirmed by immunoblotting and liquid chromatography–mass spectrometry (LC–MS/MS) analyses. An in silico allergenicity assessment suggested that all identified cashew PR10 proteins are potentially allergenic and may represent three different isoallergens.