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Flavin oxidation in flavin‐dependent N ‐monooxygenases
Author(s) -
Robinson Reeder M.,
Klancher Catherine A.,
Rodriguez Pedro J.,
Sobrado Pablo
Publication year - 2019
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1002/pro.3487
Subject(s) - flavin group , chemistry , stereochemistry , monooxygenase , hydroxylation , kinetic isotope effect , active site , substrate (aquarium) , photochemistry , enzyme , biochemistry , cytochrome p450 , biology , physics , deuterium , quantum mechanics , ecology
Siderophore A (SidA) from Aspergillus fumigatus is a flavin‐containing monooxygenase that hydroxylates ornithine (Orn) at the amino group of the side chain. Lysine (Lys) also binds to the active site of SidA; however, hydroxylation is not efficient and H 2 O 2 is the main product. The effect of pH on steady‐state kinetic parameters was measured and the results were consistent with Orn binding with the side chain amino group in the neutral form. From the pH dependence on flavin oxidation in the absence of Orn, a p K a value >9 was determined and assigned to the FAD‐N5 atom. In the presence of Orn, the pH dependence displayed a p K a value of 6.7 ±0.1 and of 7.70 ±0.10 in the presence of Lys. Q102 interacts with NADPH and, upon mutation to alanine, leads to destabilization of the C4a‐hydroperoxyflavin (FAD OOH ). Flavin oxidation with Q102A showed a p K a value of ~8.0. The data are consistent with the p K a of the FAD N5‐atom being modulated to a value >9 in the absence of Orn, which aids in the stabilization of FAD OOH . Changes in the FAD‐N5 environment lead to a decrease in the p K a value, which facilitates elimination of H 2 O 2 or H 2 O. These findings are supported by solvent kinetic isotope effect experiments, which show that proton transfer from the FAD N5‐atom is rate limiting in the absence of a substrate, however, is significantly less rate limiting in the presence of Orn and or Lys.