Premium
Protein–protein interactions controlling interfacial aggregation of rhIL‐1ra are not described by simple colloid models
Author(s) -
Sorret Lea L.,
DeWinter Madison A.,
Schwartz Daniel K.,
Randolph Theodore W.
Publication year - 2018
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1002/pro.3382
Subject(s) - ionic strength , colloid , virial coefficient , chemistry , dynamic light scattering , static light scattering , rheology , rheometry , chemical engineering , viscoelasticity , protein aggregation , aqueous solution , chemical physics , materials science , thermodynamics , nanoparticle , composite material , nanotechnology , organic chemistry , biochemistry , physics , engineering
Abstract We investigated the effects of protein–protein interaction strength on interfacial viscoelastic properties and aggregation of recombinant human interleukin‐1 receptor antagonist (rhIL‐1ra) at silicone oil–water interfaces. Osmotic second virial coefficients determined by static light scattering were used to quantify protein–protein interactions in bulk solution. Attractive protein–protein interactions dominated at low ionic strengths and their magnitude decreased with increasing ionic strength, in contrast to repulsive interactions that would be expected based on uniformly charged sphere models. Interfacial shear rheometry was used to characterize rhIL‐1ra interfacial layers. More attractive protein–protein interactions in bulk solution correlated with stronger interfacial gels. Thioflavin‐T fluorescence measurements indicated that the intermolecular β‐sheet content of rhIL‐1ra incubated in the presence of silicone oil–water interfaces correlated with gel strength. Siliconized syringes were used to probe the effects of mechanical perturbation of the interfacial gel layers. When rhIL‐1ra solutions in siliconized glass syringes were subjected to end‐over‐end rotation, monomeric rhIL‐1ra was lost from solution, and particles containing aggregated protein were released into the bulk aqueous phase. The loss of monomeric rhIL‐1ra in response to mechanical perturbation was highest under the conditions where the strongest gels were observed. Aggregation of rhIL‐1ra was strictly interface‐induced and growth of aggregates in the bulk solution was not observed, even in the presence of particles released from silicone oil–water interfaces.